Identification of Escherichia coli O114 O-Antigen Gene Cluster and Development of an O114 Serogroup-Specific PCR Assay
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چکیده
منابع مشابه
Identification of Escherichia coli O172 O-antigen gene cluster and development of a serogroup-specific PCR assay.
AIM To characterize the locus for O-antigen biosynthesis from Escherichia coli O172 type strain and to develop a rapid, specific and sensitive PCR-based method for identification and detection of E. coli O172. METHODS AND RESULTS DNA of O-antigen gene cluster of E. coli O172 was amplified by long-range PCR method using primers based on housekeeping genes galF and gnd Shot gun bank was constru...
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Enterohemorrhagic Escherichia coli O145 strains are emerging as causes of hemorrhagic colitis and hemolytic uremic syndrome. In this study, we present the structure of the E. coli O145 O antigen and the sequence of its gene cluster. The O145 antigen has repeat units containing three monosaccharide residues: 2-acetamido-2-deoxy-D-glucose (GlcNAc), 2-acetamidoylamino-2,6-dideoxy-L-galactose, and ...
متن کاملOutbreak of infantile gastro-enteritis caused by Escherichia coli O114.
Jacobs, S. I., Holzel, A., Wolman, B., Keen, J. H., Miller, V., Taylor, J., and Gross, R. J. (1970). Archives of Disease in Childhood, 45, 656. An outbreak of infantile gastro-enteritis caused by Escherichia coli 0114. An outbreak of infantile gastro-enteritis occurred at Booth Hall Children's Hospital as part of a general incident in north-western England, caused by Esch. coli 01 14K9OH2. The ...
متن کاملSequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes.
Shiga toxin (Stx)-producing Escherichia coli strains of serogroup O111 are the most frequently isolated non-O157 strains causing outbreaks of gastroenteritis with hemolytic-uremic syndrome. The O111 O-antigen gene cluster had been cloned and about half of it has been sequenced; we have now sequenced the remainder of the gene cluster, which is 12.5 kb in length and which comprises 11 genes. On t...
متن کاملCharacterization of Escherichia coli O86 O-antigen gene cluster and identification of O86-specific genes.
Escherichia coli O86 belongs to the enteropathogenic E. coli (EPEC) group, some strains of which are pathogens of humans, wild birds and farm animals. The O-antigen gene cluster of E. coli O86 was amplified by long-range PCR using primers based on the housekeeping genes galF and gnd, and then sequenced. Genes involved in GDP-Fuc and N-acetyl-galactosamine (GalNAc) synthesis and genes encoding g...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2004
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.42.8.3799-3804.2004